Proteus has developed new cellulolytic cocktails for the lignocellulose biomass hydrolysis in collaboration with the French Institut Français du Pétrole & Energies Nouvelles (IFP-EN).

CEP cellulase cocktails contain several enzymatic activities (endoglucanases, cellobiohydrolases, b-glucosidase, exoglucanases, xylanases, arabinofuranosidases…) that are particularly efficient due to impressive improvements obtained by Proteus L-shufflingTM technology.

The activities improvements allow very rapid hydrolysis combined to highest conversion yields.

INTEREST IN BIOGAZ

For biogas with cellulosic inputs, the impact of CEP cocktails are: increase in CH4 production from +15 to +30% by acceding to the initially unreachable fermentable sugars, decrease in input viscosity, decrease in the digestats, and increase the productivity by 10% minimum.

INTEREST IN FERMENTATION PROCESSES

For fermentation processes with cellulose residues, it allows to the micro-organisms to accede to the fermentable single sugars (C5 and
C6) and finally to improve the global yield and obtain higher productivity. The expected benefits using CEP cocktails are:

  • Yield improvements in case of residual cellulose in the raw materials.
  • Process Aid ( lower viscosity)
  • DSP facilitating

Contact us to organize lab or pilot trials on your feedstock.

Optimized cellulolytic cocktail for 2nd generation ethanol

chemicals-L-shuffling-scheme

Initial parameters:

Beta-glucosidase activity improvement after 2 rounds of directed evolution using L-Shuffling

After 2 rounds of L-ShufflingTM, the specific activity of the β-glucosidase was improved 242 times